15N NMR study of a mixture of uniformly labeled tRNAs.
نویسندگان
چکیده
15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 79 16 شماره
صفحات -
تاریخ انتشار 1982